| Protein name |
Monellin |
| Host compatibility |
Komagatella phaffi (Pichia pastoris) |
| Yield |
2.71 g/L (Jia et al., 2017)
|
| Scale (in specific example) |
Lab scale in bioreactor (Jia et al., 2017)
|
| Biggest scale product has been develloped in this organism |
Oobli has recently recvieved GRAS status for the production of monellin in K.phaffi, indicating plans to produce at pilot or industrial scale
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
FDA GRAS approval in the US. Not allowed in Canada
|
| Companies producing this protein in this organism |
Oobli
|
| Extra/remark |
|
| Publications/references |
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Jia, L., Tu, T., Huai, Q., Sun, J., Chen, S., Li, X., Shi, Z., & Ding, J. (2017). Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency. PLoS ONE, 12(10), e0184602. https://doi.org/10.1371/journal.pone.0184602
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Asr, S. Y., Yüksel, N., İçi̇Er, S., & Türköz, B. K. (2022). Tatlı Bitkisel Proteinler ve Rekombinant Üretimleri. TüRk Doğa Ve Fen Dergisi :/TüRk Doğa Ve Fen Dergisi, 11(3), 186–194. https://doi.org/10.46810/tdfd.1027978
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Farag, M. A., Rezk, M. M., Elashal, M. H., El-Araby, M., Khalifa, S. a. M., & El-Seedi, H. R. (2022). An updated multifaceted overview of sweet proteins and dipeptides as sugar substitutes; the chemistry, health benefits, gut interactions, and safety. Food Research International, 162, 111853. https://doi.org/10.1016/j.foodres.2022.111853
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Novik, T. S., Koveshnikova, E. I., Kotlobay, A. A., Sycheva, L. P., Kurochkina, K. G., Averina, O. A., Belopolskaya, M. V., Sergiev, P. V., Dontsova, O. A., Lazarev, V. N., Maev, I. V., Kostyaeva, M. G., Eremeev, A. V., Chukina, S. I., & Lagarkova, M. A. (2023). Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry. Foods, 12(22), 4065. https://doi.org/10.3390/foods12224065
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Zeece, M. (2020). Flavors. In Elsevier eBooks (pp. 213–250). https://doi.org/10.1016/b978-0-12-809434-1.00006-2
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Saraiva, A., Carrascosa, C., Ramos, F., Raheem, D., Pedreiro, S., Vega, A., & Raposo, A. (2023). Brazzein and Monellin: Chemical Analysis, Food Industry Applications, Safety and Quality Control, Nutritional Profile and Health Impacts. Foods, 12(10), 1943. https://doi.org/10.3390/foods12101943
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Kim, I. H., & Lim, K. J. (1996). Large-scale purification of recombinant monellin from yeast. Journal of Fermentation and Bioengineering, 82(2), 180–182. https://doi.org/10.1016/0922-338x(96)85046-x
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